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Microcentrifuge for 5 min. Incubate substrate with membrane thaiilande 1 minute, remove excess solution membrane remains wet Agence de rencontre thailande, wrap in plastic and expose to X-ray film. This can be done by re-exposing the blot to ECL reagents and making sure there is no signal prior to adding the next primary antibody. Volumes are for 10 cm x 10 cm cm2 of membrane; for different sized membranes, adjust volumes accordingly. Wrap in plastic wrap and expose to x-ray film.
Reprobing can be a valuable method but with each reprobing of a blot there is potential for increased background signal.