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These techniques comprise the detection of viral antigens by immunofluorescence and immunoperoxidase techniques and methods of viral antibody and antigen determination by the enzyme immunoassay technique. Despite these advances, significant challenges remain in the clinical management of dengue-infected patients, especially in the absence of reliable biomarkers that provide an effective prognostic indicator of severe disease progression.
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A number of general and specific recommendations are made. PubMed Central This Memorandum evaluates rapid laboratory techniques for the diagnosis of viral infections, especially with a view to their use in developing countries. Furthermore, there is no single, definitive diagnostic biomarker that is present across the entire period of patient presentation, particularly in those experiencing a secondary dengue infection.